Principal investigator, Dr. Hung-Yin Lin, earned his Ph.D. (with Prof. James L. Thomas) from Columbia University in the City of New York in 2003, and joined Prof. Tse-Chuan Chou’s team at National Cheng Kung University (Taiwan) with the funding of Ministry of Education of Academic Promote Excellent Program from 2003 to 2005. He joined National University of Kaohsiung (Taiwan) in 2005 as an assistant professor in Chemical Engineering and Material Engineering Department and adjunct assistant professor in Biotechnology Institute since 2006.

I. Biomolecular Detection
Recently, Dr. Lin et al. developed the technique of microcontact printing, which can prevent the problem of protein solubility in the solvent, when synthesizing MIPs. Microcontact printing is a significant advance in MIPs synthesis technology, as it not only requires less protein target, but also can offer an almost flat recognition surface for assembly with microfluidic system. We believe that this molecular imprinting technique could offer a tailor-made artificial antibody for the sensing of biomarker not only for cancer markers but also for viruses.
A list of publications about Artificial Receptors/Antibodies and Biocompatible Polymer Thin Films which are directed related to this research.
1. Hung-Yin Lin, Chung-Yi Hsu, James L. Thomas, Shu-E Wang, Hsiao-Chi Chen and Tse-Chuan Chou,”The Microcontact Imprinting of Proteins: The Effect of Cross-linking Monomers for Lysozyme, Ribonuclease A and Myoglobin,” Biosensors and Bioelectronics, 22, pp. 534-543, 2006.
2. Chung-Yi Hsu, Hung-Yin Lin, Bu-Tan Wu and Tse-Chuan Chou,” Styrene-containing Molecularly Imprinted Polymers Enhance the Recognition of Ribonuclease A,”Biosensors and Bioelectronics, 22, pp. 355-368, 2006.
3. Chung-Yi Hsu, Hung-Yin Lin and Tse-Chuan Chou,” Synthesis of and Recognition by Ribonuclease A Imprinted Polymers,”Nanotechnology,pp. S77-S83, 2006.
4. Mei-Hwa Lee, James L. Thomas, Shin-Ber Tasiand Hung-Yin Lin*,“Formation and Recognition Characteristics of Albumin- imprinted Poly(Ethylene-co-Vinyl-Alcohol) Membranes,” Journal of Nanoscience and Nanotechnology, in press, 2007.(NSC 95-2614-B-390 -001 -MY2 and NSC 96-2220-E-390-001-)
5. Mei-Hwa Lee, Tain-Chin Tsai, James L. Thomas and Hung-Yin Lin*,“Recognition of Creatinine by Poly(Ethylene-co-Vinyl Alcohol) Molecular Imprinting Membrane,” Desalination, in press, 2007. (NSC 96-2220-E-390-001-)

II. Controlled Release Nanocarriers
Ultrasound is commonly used in the preparation of unilamellar liposome dispersions, and is often considered for drug delivery or DNA transfection applications. To better understand the physical and chemical properties of lipid membranes that render them susceptible to ultrasonic permeabilization, the roles of temperature, lipid composition (cholesterol and PEG-lipid content) and liposome size have been studied. The results of these studies suggest that lipid packing is very important to ultrasound responsiveness; surprisingly, cohesive energy and tensile strength are not. Taken together, the experimental results implicate a defect-mediated permeabilization mechanism, rather than pore formation or membrane tearing.
A list of publications about Ultrasound Sensitive Nanocarrier (liposomes and microemulsions) and the Encapsulation with Biomolecules which are directed related to this research.
1. Hung-Yin Lin and James L. Thomas,” PEG-Lipids and Oligo(ethylene glycol) Surfactants Enhance the Ultrasonic Permeabilizability of Liposomes,” Langmuir, Vol. 19, pp. 1098-1105, 2003.
2. Hung-Yin Lin and James L. Thomas,” Factors Affecting Ultrasound Responsivity of Unilamellar Liposomes,” Langmuir, Vol. 20, pp. 6100-6106, 2004.
3. Mei-Hwa Lee, Hung-Yin Lin, Hsu-Chih Chen and James L. Thomas,” Ultrasound Mediates the Release of Curcumin from Microemulsions,” Langmuir, Vol. 24, pp. 1707-1713, 2008.
4. StavroulaSofou, James L. Thomas, Hung-Yin Lin, Michael R. McDevitt, David A. Scheinberg and George Sgouros, “Engineered Liposomes for Potential Alpha-Particle Therapy of Metastatic Cancer,” Journal of Nuclear Medicine, Vol. 45, No. 2, pp. 253-260, 2004.
5.James L. Thomas, Hung-Yin Lin and Natalia Rapoport,” Responsive Liposomes for Ultrasonic Drug Delivery”, US Patent, 2006. (#20060002994)